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Briefly, adult fat bodies were dissected in 1 phosphate buffered saline (PBS), fixed 20 min in 4% paraformaldehyde at room temperature (RT), rinsed 5 in 1 PBS, then permeabilized for 30 min at RT in 1xPBS + 0.3% Triton X-100

10.1093/sexmed/qfae018 17 GiraseT.PatilJ.TatiyaA.PatilD.PatilM

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Indirect calorimetry and locomotor activity were continuously monitored using a comprehensive lab animal monitoring system (CLAMS