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The inert chemistry of the MSIA tips provide reduced nonspecific binding, lowering background and minimizing the work load on LC systems, which contribute to increased signal-to-noise and improved assay sensitivity, as shown in Figure 2

Prepared cDNA was used in the qPCR analyzer (StepOne, Applied Biosystems, Singapore) using MAXIMA SYBR Green qPCR Master Mix with the following program: 1 cycle at 95 C for 10 min

Thus, identifying the molecular substrates underlying these early adaptations is essential for understanding eCB-stress interactions and informing novel therapeutic strategies

But behind every number is a structural decision

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