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Briefly, each animal was placed in a rat cage containing bedding with a familiar odor (from the home cage) on one end and sterilized odorless bedding on the opposite end
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The luciferase activity measurements were performed by transferring 72 L of assay buffer (25 mM glycylglycine, 5 mM KPO 4 , 50 mM MgSO 4 , 10 mM EGTA, 2% ATP, 1 mM dithiotreitol) to a white 96-well plate and adding 20 L of the cell-lysis suspension with subsequent adding of 40 L D-Luciferin and immediate measuring using the Berthold LB960 Centro XS3 plate luminometer
Approximately 30 minutes plasma half-life
The differential survival rate between cells unexposed and exposed to UV radiation was then calculated, and the results were expressed as percentages of dead cells after UV exposure
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