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MEGA-PRESS may be a promising protocol, although the current MEGA-PRESS studies reporting brain GSH in MCI and AD were from a single research group, which may have artificially reduced heterogeneity

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Pellets were resuspended in DMEM/F-12 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 20% foetal bovine serum (FBS, Pan Biotech, Aidenbach, Germany) and penicillin/streptomycin (Life Technologies) and plated on 10 cm cell-culture dishes

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21 Possible role of the GPX4 gene in the development of CS A possible role of glutathione peroxidase-4 in the development of CS has been summarized in Figure 1