After adding EnVision Flex 3,3-diaminobenzidine tetrahydrochloride (DAB, K346811-2, Agilent, Santa Clara, CA, USA) for 5 min at room temperature to visualize GPx3, the slides were washed with tap water, counterstained with hematoxylin and mounted in Pertex
Brandon Richland, MD.
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Scalp defects in infants of mothers treated for hyperthyroidism with methimazole or carbimazole during pregnancy
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ALDH activity was determined by monitoring the formation of NADH at 340 nm during the oxidation of propionaldehyde as described 55