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In all cases, motif enrichment in regions upstream of differentially expressed genes was assessed relative to a background gene set that included only liver-expressed genes not identified as differentially expressed

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For example, the most prominent alteration in the liver of the MTX-treated rats was congestion of the central vein, focal hepatocyte necrosis with loss of lobular pattern, nuclear lysis, and Blood sinusoids are dilated and full with mononuclear inflammatory cells