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Pellets were thawed and resuspended in purification buffer (150 mM NaCl, 2 mM MgCl 2 , 50 mM HEPES-KOH pH 7.5, 5 mM -mercaptoethanol, 10% (v/v) glycerol) supplemented with protease inhibitors and DNase I before lysis by sonication
Friedmann Angeli et al., 2014
Make an appointment with a health care professional if you can't pinpoint the reasons for a late period, as your provider may want to check for specific health conditions
Symptoms include joint swelling (particularly the big toe), pain, redness, and heat
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