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Step 5: Store in the refrigerator inside the black box provided by the clinic

Protocols vary based on the condition being treated acute injuries typically involve a more intensive initial phase, followed by a maintenance dose

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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26