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Oxidation kinetics for roGFP2 WT -PfGrx fusion constructs The in vivo oxidation kinetics from fusion constructs between roGFP2 WT and a variety of glutaredoxins and mutants thereof were shown to correlate very well with the rate constants for the oxidative half-reaction from GSSCys assays in vitro, suggesting that the glutathionylation of the glutaredoxin and not the transfer of oxidation to the fused roGFP2 moiety was rate-limiting under the chosen conditions 15,34,35
Broad gene-mapping work led by Pickart and colleagues reported modulation of thousands of genes, with roughly a third of human gene patterns shifting toward expression states associated with younger or healthier tissue
The three non-standard residues are central to its selectivity at GHS-R1a
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