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Core hypothalamic tissue dissection For the RNA-Seq experiment, core hypothalamic tissues of 5-week-old (pre-obesity) and 12-week-old (3 Bbs5 / and 2 WT littermates per age group) mice were collected using a brain tissue slicer and micropuncher (Integra-Miltex)

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A vial stored at 6C for 28 days delivers consistent results

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