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19 Immunohistochemistry For paraformaldehyde (PFA) fixation, mice were deeply anesthetized with isoflurane and perfused sequentially with heparinized normal saline, followed by 4% PFA and 0.5% glutaraldehyde
Thorax 64, 6773
Requirements usually include: (1) BMI 30 , or BMI 27 with at least one weight-related health condition (type 2 diabetes, high blood pressure, or high cholesterol per FDA labeling), (2) participation in a 6-month weight management program (diet, exercise, and lifestyle changes), (3) chart notes documenting BMI and health conditions, and (4) no contraindications such as personal/family history of medullary thyroid cancer or MEN2 syndrome (a rare genetic condition)
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