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Indeed, despite attempts to generate a egtA gliK mutant on multiple occasions, using a variety of strategies, no successful egtA gliK mutant was generated (unpublished observations), which indicates that the simultaneous absence of EgtA and GliK is not possible in A
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For thiols to show up in a glass of IPA, concentrations need to exceed 100 ng/L, and that doesnt happen with traditional or hybrid strains
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