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DNA interactions were studied under laboratory conditions

For optimization targets and testing comparisons, we considered all available datasets from previously published plasticity experiments for glutamatergic connections in the juvenile rodent neocortex that used whole-cell paired recordings: layer 5 thick-tufted pyramidal cell (L5-TTPC) to L5-TTPC 12,14 , layer 2/3 pyramidal cell (L2/3-PC) to L5-TTPC 36 , L2/3-PC to L2/3-PC 41,43,44 , layer 4 pyramidal cell (L4-PC) to L2/3-PC 42 , layer 4 spiny-stellate cell (L4-SSC) to L4-SSC 41 (see Table 1)

Room temperature storage often coincides with light exposure because refrigerators provide dark environments while countertops and shelves do not

Substituting one form for another should only happen with professional guidance

Not for use in newborns due to benzyl alcohol toxicity profile in neonates

Cells were washed with PBS and blocked with 5% BSA for 1 h at RT