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While these results demonstrate that further negative selection reduced off-target cleavage, all three variants performed worse than X(PC) at cleaving full-length procaspase-1 in mammalian cells
331 Oxidative stress is a characteristic feature of chronic kidney disease, initiating inflammation and endothelial dysfunction, which accelerates arteriosclerosis
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The pooled phage output surviving PACE on PC_SS1 was next subjected to phage-assisted non-continuous evolution (PANCE) 52,53,54 on PC_SS2, which contained six additional mutations toward procaspase-1 (Fig
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