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Peptides in solution are far less stable than in their dry, lyophilized form, so how and where you keep the vial directly determines how long the material remains intact

V., Thoroughgood, C

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For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio

Toxicology 372, 2233

Furthermore, neutrophils from lupus-prone mice or Systemic Lupus Erythematosus (SLE) patients undergo ferroptosis, which leads to the disruption of immune tolerance in SLE