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10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation
Subsequently, lysates were centrifuged at 12,000 rpm for 15 min at 4 C to obtain clear supernatants
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The hallmarks of cancer metabolism: still emerging
Before diving into the current status of human studies, it's important to summarize key findings from animal models: These studies suggest a broad healing profile, but animal results don't always translate directly to humans
