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There are other alkylating agents used earlier for masking GSH in glutathione assay like N-ethyl-maleimide, 2-Vinylpyride etc
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The bound protein was eluted using 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, and 5 mM EGTA and 0.1 mg/mL Flag peptide
Freezing at -20C or below keeps the effectiveness for a long time, but repeated freeze-thaw cycles cause aggregation, which can be prevented by separating the samples
Semaglutide is a peptide medication made of a synthesized glucagon-like peptide (GLP-1)
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