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To assess the effects of fibrotic agonists on IGF-1/IGF-1R expression in cardiac cells, we infused C57BL6 mice with AngII and phenylephrine (AngII/PE) through osmotic minipumps for 7 days and then isolated and plated cardiomyocytes and cardiac fibroblasts
For studies in DIO mice, male C57BL/6J mice were fed with a high-fat diet comprising 58% kilocalories from fat (D12331, Research Diets)
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