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10.1080/1028415X.2020.1853417 72 MikulskaJ.JuszczykG.Gawroska-GrzywaczM.HerbetM

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Gene-gene interactions Table 1 clearly shows that many authors reported an increased maternal risk of birth of a child with DS for carriers of combined genotypes given by the presence of two simultaneous risk alleles, mainly combinations of MTHFR 677T and 1298C alleles, or combinations of MTHFR 677T and MTRR 66G ones (Table 1)

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this discrepancy is reasonable considering the yield of HGF after a wash-out step that often causes trapping/stacking of proteins on ultra-filtration membranes