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To determine whether this mutation affected the intracellular ATP content, the ATP pool was quantified and compared to MG1655 cultured in M9 medium (Fig

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doi:10.1016/j.mad.2003.11.005 Huff T, Mller CS, Otto AM, Netzker R, Hannappel E

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Fluorescence spectra were acquired using an FS5 spectrofluorometer (Edinburgh Instruments) equipped with an SC-40 plate reader and 96-well plates (Corning) using Fluoracle software

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