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3) sirtuins, main sensors for nutrient state, are activated by increased NAD + /NADH ratio 12
The maXis II ETD mass spectrometer (Bruker) was operated with the captive source in positive mode employing the following settings: mass range: 2002000 m/z , 2 Hz, capillary 1600 V, dry gas flow 3 L/min at 150 C, nanoBooster 0.2 bar, precursor acquisition control set to fragment the top 20 most abundant peaks
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