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I gained three pounds

RNA isolation and qRTPCR analysis Total RNA was extracted using TRIzol (Takara Bio, Tokyo, Japan) and quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed using Hieff qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) according to the manufacturers instructions

Optimal Dosages and Sources of L-Carnitine for PCOS Assessing the Right Dosage of L-Carnitine for PCOS Determining the ideal amount of L-carnitine for PCOS is key