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taking certain antibiotics) that reduce carnitine absorption or increase its excretion

The cells were washed twice with PBS, 400 l of PBS were added to each well, and fluorescence intensity was determined with a flow cytometer (analyzed by FlowJo 7.6.1)

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Advancement of these hits into biochemical PDE inhibition assays and early ADME characterization (including aqueous solubility, microsomal stability, and P-gp interaction assessment) is essential to determine whether the proposed binding modes translate into quantifiable enzymatic potency and brain-penetrant pharmacokinetic profiles (Soon et al., 2024)