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Her approach is attentive, engaged, and calming
Cells were washed with PBS and imaged in 11 mM glucose KRBH buffer using a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices) with a 20 air objective and 488 nm laser, with images taken every 6 seconds, on a 37 C stage
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NsPCS were dissolved in Milli-Q water and adjusted to a concentration of 220 mg/L
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