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S4 The calibration curve of R6G solution
Under carbon dioxide anesthesia, blood samples were withdrawn from the orbital sinus into K2EDTA-treated tubes and centrifuged at 2000 g for 15 min at 4C to obtain the plasma fractions and stored at 20C
Always use airtight, light-resistant containers for storage
The feeding medium contained 500 g/L glucose and 25 g/L MgSO 4 7H 2 O
The preparation was fixed in a solution containing acetic acid and ethanol (1:3) for 5 days
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