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Three weeks later, mice were terminally anaesthetised with 100 mg/kg pentobarbital and transcardially perfused with PBS containing 4% PFA, brains were collected, post-fixed in 4% PFA at 4 C overnight and cryoprotected in 30% sucrose for 23 days
The doses used in those models, when allometrically scaled to humans using a standard body surface area conversion, suggest a range of roughly 200 to 500 mcg per day
Responses to the less chartered topics will provide an insight into better and safer clinical practices