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Key safety principles include strict sterile technique to prevent contamination and infection, proper injection site preparation and rotation, correct needle insertion angle and depth for chosen administration route (IM or SC), slow administration to minimize discomfort, and appropriate post-injection site care and monitoring

Methods Eligibility criteria The PICOS strategy was defined as follows: P (participants) human subjects, I (interventions) oral LC treatment, C (comparisons) between supplementation and placebo, supplementation and control, or pre- and post- supplementation, O (outcomes) muscle variables, and S (study design) randomized controlled trials, non-randomized controlled trials, non-randomized non-controlled trials

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AAPH addition cannot be performed simultaneously in all the 96 wells and, in fact, it takes approximately 90-150 s to dispense it carefully in all of them, even if a well-experienced lab analyst uses a multi-channel pipette or the multi-detection plate reader includes an automatic injector

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