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To determine which deaminase contributes to the deamination of glutamine under methionine semi-starvation, the transcription levels of the four genes were compared in different cultures, no methionine and 10 g/mL glutamine (M0Q10), no methionine and 100 g/mL glutamine (M0Q100), and 200 g/mL methionine and 100 g/mL glutamine (CDM)

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Water molecules were modelled manually into clear spherical density within reasonable hydrogen bonding distance to amino-acids or other waters