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DMSO is a strong inducer of DNA hydroxymethylation in pre-osteoblastic MC3T3-E1 cells
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PMID 25585598
Another factor is reproducibility
Some researchers find larger volumes uncomfortable, and the storage duration becomes a consideration since you are pulling more liquid from the vial each week
Insert the needle into the pinch at a 4590-degree angle (use 45 degrees if the fat layer is thin) [14]
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