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Gradient separation was performed with water and 0.2% formic acid (Thermo Fisher Scientific, Waltham, MA, USA) as mobile phase A, and acetonitrile with 0.2% formic acid as mobile phase B

if the SSA concentration is 1% or more, chromogenic inhibition will occur

Despite the accumulating evidence around pathways engaged by the GLP-1R and other GPCRs in target tissues, there is still substantial uncertainty about the precise molecular mechanisms engaged, particularly with regards to the spatiotemporal organisation of their signalling and presence in spatially restricted subcellular (and even suborganelle) signalling hubs or nanodomains 61

We then tested ATP abundance by liquid chromatographymass spectrometry (LCMS) and show increased ATP levels in cells that survive 5FU treatment (Fig