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To assess whether RT probe binds to any proteins or reacts with free cysteine residues in some proteins that may interfere with the final ratio readouts, we incubated RT with cell lysate, which was dialyzed using a 3 K cut-off membrane to remove all small molecules including GSH, and observed no significant changes in fluorescent signal ratios even at the highest lysate concentration shown (protein concentration is 36 mg ml 1 based on a BCA assay), suggesting that there is no non-specific interactions or reactions between cellular proteins and RT which could significantly change the ratio readouts (Fig

It is not formulated for diagnostic or therapeutic implementation

& Riley, P

Patricks work has laid the groundwork for further exploration into the potential therapeutic uses of NAD+ precursors

Therefore, individuals with active cancer should not use it without prior consultation with a healthcare provider, even if it is sometimes used as an adjunct in cancer treatment

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