The animal models behave normally without signs for pain, stress, or discomfort
Natural 13 C abundance and tracer impurity were addressed with correction calculations using the IsoCorrectoR R-package 82 and normalized to total metabolite intensity
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These cells were cultured in Dulbeccos modified medium (Wako) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Wako) under 5% CO 2 at 37C
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It is naturally present in human blood, saliva, urine, and its plasma concentration declines with age from approximately 200 ng/mL at age 20 to under 80 ng/mL after age 60