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10.1074/jbc.M116.723700 81 McElroyP

In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

Enter the liquid volume added (mL)

Most experienced researchers use both methods

While many sleep compounds target GABA receptors (promoting sedation), DSIP is studied for its potential to promote natural sleep architecturespecifically increasing slow-wave sleep without necessarily causing sedation

For additional polar metabolite analysis, a HILIC method was used, with an Xbridge amide column (100 2.1 mm internal diameter, 3.5 m