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Generation of GFP-tagged cell lines HepG2-GFP reporter cell lines expressing tagged Nrf2, HMOX1, or SRXN1 were constructed with bacterial artificial chromosomes that encode C-terminal GFP-tagged fusion proteins as described previously (Wink et al

Under deep anaesthesia half of the animals were then perfused with ~ 5 ml PBS followed by 4% PFA, brain and spinal cord were collected for IHC analysis

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When adverse effects occur, they are typically mild and may include: Nausea or stomach upset Diarrhea or loose stools (particularly at higher doses) Dizziness Headache Fatigue These effects are usually dose-dependent and resolve with dosage adjustment