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Two defense-related genes, encoding a GST and a cytochrome P450, were regulated downstream of WRKY45, but were not regulated by NH1, suggesting independence of the WRKY45 and NH1 pathways (Shimono et al., 2007)
An assay that lacks sensitivity at low cell numbers or introduces variability between plates, will undermine every downstream result
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During Phase 1 detoxification, a family of enzymes known as Cytochrome P450 (CYP450) modifies toxins through oxidation
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