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From a mechanistic perspective, recognizing a metabolic subtype is clinically informative because it frames reproductive dysfunction as potentially downstream of systemic metabolic stress acting on the hypothalamicpituitaryovarian (HPO) axis, thereby prioritizing metabolic risk reduction as a plausible lever for improving reproductive function and for tailoring treatment intensity

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The animals were kept under a 12-hour light/dark cycle at a temperature of 25C

NOGUCHI: The equivalent of a five-foot-three person weighing 90 pounds

To make the hEC surface charge more positive the glycocalyx of hECs was digested with neuraminidase enzyme (1 U/ml, 1-h pretreatment) or cultures were treated with cationic lipid 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH