Therefore, there is a very low risk of B12 toxicity, even at high doses like 1,000 mcg a day
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In that sense, it is one of the strongest confirmatory tests for B12 deficiency
A finer gauge needle (e.g., 27G to 30G) is typically preferred for drawing solvent and carefully injecting it into the peptide vial to minimize damage to the rubber stopper and reduce potential for contamination
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Frontiers in Pharmacology, 7, 119