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Specifically, inhibiting or depleting spinal microglia abolished the anti-allodynic effects of morroniside, while neutralizing IL-10 or -endorphin with targeted antibodies, or blocking the -opioid receptor, fully reversed morroniside-induced mechanical anti-allodynia [74]
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Rats were initially anesthetized using thiopental sodium (100 mg/kg body weight, intraperitoneal injection) following established protocols [28]
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