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Frozen cortical samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail

Depending on your product, unmixed (dry) methylcobalamin vials should be stored following product instructions

The second author thanks the Ministry of Health Malaysia for providing a fellowship grant entitled Hadiah Latihan Persekutuan (HLP) for her role in this study

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Consult your healthcare provider before use, especially if you are taking any medications or other supplements as there may be potential interactions