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This discrepancy may reflect formulation challenges arising from the intrinsic instability of antioxidants

The absence of preservatives makes sterile water safer for direct tissue contact and reduces the risk of irritation or adverse reactions

Similarly, assessing the effects on lungs and brain helps determine if AgNPs might pose risks in these sensitive areas (Suresh et al., 2019)

Declarations Authors contributions Designed the paper, selected and prepared the references, prepared the figures, wrote the manuscript: Wright JW Prepared the references, wrote the manuscript: Harding JW Availability of data and materials Not applicable

Changes of 10% or more represent meaningful progress